Antineoplastic Activities of Grape Seed Proanthocyanidin Extract against Ehrlich Solid Tumor Bearing Mice Induced Alterations in AFP, CEA, TNF-α and DNA Damage
Massoud Ahmed
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
Tousson Ehab *
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
Said Noor Eldin Ahmed
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
Elwan M. Mona
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
Hameed M. Haneen
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background and Objective: Cancer is initiated due to abnormalities in the DNA of the affected cells leading to an extra mass of tissue termed a tumor. Breast cancer is the most public cancer amongst women world-wide. The present work is designed to investigate the ameliorating potential effects of grape seed proanthocyanidin extract (GSPE) in inhibition of Ehrlich cells growth and tumor development as a model for breast cancer. Also; the present work is designed to investigate the effect of GSPE on the changes in the levels of AFP, CEA, TNF-α and hematological alterations, and DNA damage examination on Ehrilch solid tumor (EST) bearing mice.
Materials and Methods: A total of 40 female mice were evenly distributed amongst four groups (G1, control group; G2, GSPE group; G3, EST group; IV, EST+GSPE group).
Results: Results revealed increased incidences of tumor growth in the untreated EST group, along with elevated levels of serum AFP, CEA, TNF-α, WBCs, platelets and DNA damage and an associated reduction in RBCs and Hb%. Treatment of EST with GSPE (EST+GSPE) modulates and improved these changes in CBC, tumor markers and DNA damage as compared to mice bearing EST.
Conclusion: This finding calls for more investigation on the benefits of grape seed proanthocyanidin extract (GSPE) as antineoplastic activity on other tumors as Ehrlich ascites carcinoma or cancer.
Keywords: Ehrlich solid tumour, GSPE, AFP, CEA, TNF-α, DNA damage, mouse.